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I have replaced the screen for my iPhone 5s never had a problem replacing screens before and the home button works when I turn the phone on to check before clicking all back into place but once it’s clicked into place it doesn’t work any help would be greatly appreciated ?

You need to closely inspect the Home Button assembly and bracket to insure everything is in its proper place. There’s not much wiggle room inside an iPhone and if something wasn’t assembled properly, then it will either physically or electrically obstruct proper function.